Antibiotic Susceptibility and Biofilm Formation of Clinical Isolates of Pseudomonas Species from Wounds Specimens

Abstract

A hallmark of Pseudomonas aeruginosa is its ability to establish biofilm based infections that are difficult to eradicate. Biofilms are less susceptible to host inflammatory and immune response and have higher antibiotic tolerance that frees living planktonic cells. The aim of the study is to investigate biofilm forming capacity and the antibiotic susceptibility profile of Pseudomonas aeruginosa strains isolated from clinical wound specimen. A total number of60 wound specimens will be submitted to the bacteriology laboratory of Abubakar Tafawa Balewa University Teaching Hospital for investigation, and will be screened for Pseudomonas aeruginosa and strains will be identified on the basis of cultural characteristics, Gram staining, biochemical tests such as citrate, urease, indole, fermentation of sugar using triple sugar agar. The biofilm forming capacity of the strains will be tested using the test tube method after the strains are standardized to approximately standard inoculated into a cooked meat broth. The growth rate of Pseudomonas aeruginosa clinical strains after 48 hours incubation will be measured by taking the absorbance using Densi-Check. The strain growth rate will be checked. Biofilm formation at the liquid interface (pllicle) will be observed as a ring and this will be qualitatively scored from the first to the last strain. The cultures will be decanted gently and rinsed twice with sterile distilled water and steained with 0.1% w/v safranin, the stain will be decolorized using 100% alcohol, and the absorbance for strains will be measured at 590nm. The strain with the most and least biofilm formation will be recorded with the absorbance rates. The clinical significance of the Pseudomonas aeruginosa biofilm forming capacity and resistance to antibiotics which could result to none healing, delayed healing, foul smell of wound infection will be checked for the experiment.