Journal of Prevention, Diagnosis and Management of Human Diseases

Role of Computed Tomography Scanner for Different Phase Study of Abdomen

2024-12-17T06:54:05+00:00

This study is aimed to see the different phase of abdomen using computed tomography in various diseases. The study will help the radiographer regarding the patient preparation and protocols are optimized for patient care and well standard. The aim is to see the hemangioma, metastasis, tumor, carcinoma, hepatic vein & portal vein. The research was made on a theoretical examination conducted as a part of daily work. Some facts were taken from many other sources and studies and some were from standard international and national books. The role of computed tomography for different phase of abdominal scan to find out various abnormalities, disease by NCCT OF ABDOMEN, CECT OF ABDOMEN, TPCT, DUAL ENERGY CT. The common cause of different disease observed in my study period includes Metastatic, HCC, Liver abscess, cirrhosis, inflammations, abnormalities, infection in intestines and cortical cyst. This study is to exhibits the common parameter that help to diagnose pathology like metastasis, liver abscess, cirrhosis, inflammations, abnormalities, infection in abdomen, intestinal infection cortical cyst, polyp in large intestine. CT examining is quick, easy, harmless, and exact. Since it can recognize very small nodules in the abdominal scans, Abdomen CT is particularly viable for diagnosing abnormalities of whole abdomen and the organs which comes under in the abdomen, and tumors at most treatable stage. A CT scan creates pictures that can be reformatted in numerous planes. It can even create three layered pictures. Our doctor can audit these pictures on a computer screen, print them on film or through a 3D printer, or move them to a CD or DVD.

Genetic Variation in Microrna-423 and Microrna 137 Genes among Patients with Breast Cancer

2024-12-24T06:25:54+00:00

Aim MicroRNAs (miRNAs) are a family of non-coding RNAs that have been suggested as novel markers or therapeutic targets for breast cancer. In current study, we aimed to investigate the prevalence of microRNA-423 rs6505162C/T and the VNTR length of the MIR 137 gene (rs58335419) with regards to breast cancer susceptibility in Iraqi women. Methods: In this study, 190 individuals took part, out of which 110 were breast cancer patients and 80 were healthy controls. The amplification refractory mutation system PCR method (ARMS-PCR) was used to determine the polymorphism in the microRNA-423 (rs6505162) gene. While the VNTR length of the MIR 137 gene (rs58335419) was identified using PCR. The Graph Pad Prism 9 software was used to conduct the statistical analysis. Results: the allele frequency of T in the microRNA-423 rs6505162 was significantly associated with breast cancer (odds ratio [95% confidence interval] 2.333 [1.519-3.600], Risk factor [95% confidence interval]) 1.400 [1.184-1.655], p < 0.0001). On the other hand, all the allele frequencies in the MIR 137 gene (rs58335419) were not found to be associated with breast cancer. Conclusion: Based on the results, the study suggests that microRNA-423 rs6505162C/T could be useful markers for diagnosing breast cancer, whereas the MIR 137 gene (rs58335419) is not useful for diagnosing breast cancer.

Gene Expression Profiling Based on High-Throughput Sequencing Technology and Its Application in Disease Diagnosis

2025-01-13T11:49:13+00:00

Pulmonary tuberculosis tends to co-occur with other diseases, leading to the emergence of drug-resistant strains of Mycobacterium tuberculosis, which complicates the identification and treatment of pulmonary tuberculosis. The limitations of previous methods have become increasingly evident. Therefore, based on high-throughput sequencing technology, this study aims to screen for diagnostic marker genes by comparing the differences in PBMC expression profiles between pulmonary tuberculosis patients and healthy individuals. During the research process, PBMCs were first isolated from samples, total RNA was extracted, and cDNA libraries were prepared for bridge PCR amplification. Subsequently, deep sequencing was performed using an Illumina Genome Analyzer IIx sequencer to determine the read counts of target genes, calibrate sequence tags for high-throughput sequencing data, screen for differentially expressed genes of PTB and HC with higher expression levels, and make diagnoses based on the condition.